Sebastiano Rolli studied Chamber Music and Composition at the Conservatory in Parma and Milan and Musical drama with Marcello Conati. He started his career as a conductor at a very young age, distinguishing as one of the most exciting performers of his generation.

In the past seasons he conducted: Macbeth at the Slovak National Opera in Bratislava; Maria Stuarda at the Teatro Sociale in Rovigo, Teatro Donizetti in Bergamo and Teatro Filarmonico in Verona; Il Trovatore, Maria di Rudenz and Torquato Tasso again in Bergamo; Falstaff at the Teatro Verdi in Busseto for the Festival Verdi; I Puritani at the Slovak National Opera in Bratislava; Gianni Schicchi and Suor Angelica in Tirana; Anna Bolena in Tenerife; Die Lustige Witwe  at the  Teatro Lirico in Cagliari; La Cenerentola and Pierino e il lupo at the Filarmonico in Verona;  Lucia di Lammermoor at the Regio in Parma and in Savona and La Sonnambula in Catania.

Most recently he conducted: Donizetti's Rosmonda d'Inghilterra in Bergamo and in Florence; Bellini's La straniera in Catania; L'Italiana in Algeri with the Opera of Florence in Oman; Norma in Tenerife; La Traviata and La Sonnambula in Florence, Parma, Busseto and Bolzano; Roberto Devereux and Un ballo in maschera in Parma; Nabucco for the Israeli Opera in Tel Aviv;Il Trovatore for the Rete Lirica delle Marche, in Tirana and in Fidenza for Festival the Verdi; Pagliacci in Sassari; Il Barbiere di Siviglia and Don Pasquale in Fribourg; Il Maestro di Cappella on tour in Italy with the Orchestra Filarmonica Italiana; Donizetti's Il giovedì grasso in Ancona; Macbeth at the Opera in Dijon; I Lombardi alla prima crociata and I Due Foscari at the Teatro La Fenice in Venice; Rigoletto in Treviso, Rovigo; Il Barbiere di Siviglia in Nancy; L'Elisir d'amore in Magdeburg.

On the concert side, he conducted a Faure's Requiem, Verdi's Quattro pezzi sacri at the  Teatro Lirico in Cagliari; recital at the Concert Hall in Dublin and a tour in Mexico and Perù along with Juan Diego Florez; Verdi's Requiem in Madesana with the Teatro Regio of Parma; concerts at the Arena di Verona Orchestra and at the Olympia Theater in Athens and with the Università Statale Milano Orchestra, where he is principal conductor.

Among his numerous publications, two critical studies on "Giuseppe Verdi. The masters of melodramma "Giuseppe Di Stefano. His characters" - Azzali Publishers. He is regularly invited by musical institutions and conservatoires to keep masters in- depth interpretation of Verdi or choral music. He was professor of interpretation of the score and vocal diction for singers in the courses "Mythos" of the "Fondazione Arturo Toscanini" and "Fondazione Teatro Alla Scala" in Milan.