Matteo Macchioni was born in Sassuolo and began studying music as a child. In 2007, he obtained an academic diploma in piano, while at the same time cultivating his study and passion for opera singing. In 2010, he made his debut at the Giuseppe Verdi Theatre in Salerno in the role of Nemorino in L'Elisir d'amore conducted by Daniel Oren.

Under the guidance of Natale de Carolis, he made his debut at the "Luglio Musicale Trapanese" Festival in the role of Ernesto in Don Pasquale and later the role of Conte di Almaviva in Il Barbiere di Siviglia at the Stresa Festival; Goro in Madama Butterfly, Don Basilio in Le Nozze di Figaro at the Teatro Carlo Felice di Genova and again Almaviva in Il Barbiere di Siviglia under Filippo Crivelli. He took part in the production of Il Viaggio a Reims at the Rossini Opera Festival in Pesaro as Cavalier Belfiore, after successfully participating in the Accademia Rossiniana presided over by Maestro Alberto Zedda.

This was followed by productions of: Il Barbiere di Siviglia at the Teatro Verdi in Padua and at the Teatro di León (Mexico); Adriana Lecouvreur (Abate di Chazeuil) on tour with the Circuito Lirico Lombardo and in Sassari; Britten's Billy Budd conducted by Andrea Battistoni and directed by Davide Livermore at the Teatro Carlo Felice in Genoa; Don Giovanni at the Teatro Olimpico in Vicenza; Pagliacci (Arlecchino) at the Festival "Como Città della Musica"; La gazza ladra (Isacco) conducted by Donato Renzetti and directed by Damiano Michieletto at the Rossini Opera Festival in Pesaro

In past seasons, noteworthy engagements include: Le Nozze di Figaro conducted by Stefano Montanari under the direction of Mario Martone/ Di Florio in the Circuito Lombardo and later at the Teatro Regio in Parma and at the Valli in Reggio Emilia; La Cenerentola at the Leipzig Opera House and at the Maggio Musicale in Florence; Olivo e Pasquale at the Donizetti Festival in Bergamo.

In 2017 he made his debut at Teatro alla Scala in Milan in Rossini's La gazza ladra conducted by Riccardo Chailly and directed by Gabriele Salvatores, and later in Mosè in Egitto at the Bregenzer Festspiele. He was again Almaviva in Il Barbiere di Siviglia at the Teatro Sociale di Rovigo and at the Royal Danish Opera House in Copenhagen and took part in the production of Adina in Pesaro. He made his debut at the Welsh National Opera in Cardiff in La Cenerentola and in L'Occasione fa il ladro Tiroler Festspiele in Erl.

More recently, he returned to the Carlo Felice in Genoa with Don Pasquale; he soon took part in the production of Don Giovanni at the Theater Freiburg and in Diano Marina; Guillaume Tell (Ruodi) in Erl and Così fan tutte at the Royal Danish Opera House in Copenhagen; Rossini's Stabat Mater and Il Barbiere di Siviglia in Trieste; Gianni Schicchi in Empoli and Matera; Don Pasquale in Rovigo and Le Nozze di Figaro and Die Zauberfloete at the Filarmonico in Verona; Il Paese dei Campanelli in Martina Franca and Novara and La Cenerentola at the Olympia Thater in Athens.

Upcoming engagements include I Puritani in Modena, Turandot (Pong) at the Arena di Verona, L'equivoco stravagante in Pesaro, Tosca (Spoletta) at the Accademia di Santa Cecilia in Rome, Daniela Harding conducting and Deutsche Grammophon recording, Cenerentola in Klagenfurt and Il Turco in Italia (Albazar) at the Filarmonico in Verona.

April 2024